Fig. 3
From: Intestinal Piezo1 aggravates intestinal barrier dysfunction during sepsis by mediating Ca2+ influx
Piezo1 plays a pivotal role in the disruption of the intestinal barrier induced by TNF-α leading to increased degradation of TJ proteins and apoptosis. A, B Immunofluorescence analysis of Piezo1 Caco-2 cell monolayers, and quantification of immunofluorescence analysis (n = 3 for each group). Scale bar, 50 μm. C–E Immunofluorescence analysis of ZO1 and Occludin in Caco-2 cell monolayers, and quantitative analysis of immunofluorescence evaluation (n = 3 for each group). Scale bar, 20 μm. F Apoptosis were analysis by PE and ANNEXIN V- FITC double staining, and apoptosis rates are displayed as histograms (n = 3 for each group)