Fig. 2
From: Intestinal Piezo1 aggravates intestinal barrier dysfunction during sepsis by mediating Ca2+ influx
Villin-Piezo1flox/flox mice demonstrate resilience against CLP-induced disruption of the intestinal barrier. A Intestinal permeability is detected by administering FITC-dextran in plasma (n = 4 for each group). B–D Immunofluorescence analysis of ZO1 and Occludin in the intestinal tissues of mice, and quantitative analysis of immunofluorescence evaluation (n = 4 for each group). Scale bar, 50 μm. E Section of intestines were subjected to TUNEL staining. Scale bar, 50 μm (n = 4 for each group). F M1 and M2 macrophages were identified with flow cytometry with markers of CD86F4/80 and CD206F4/80, respectively (n = 3 for each group)