Fig. 1

Piezo1 participates in the impairment of the intestinal barrier of CLP-induced sepsis mice. A Immunofluorescence analysis of Piezo1 in the intestinal tissues of mice with normal conditions and mice with sepsis. Scale bar 50 μm. B Quantitative analysis of immunofluorescence evaluation was conducted (n = 4 for each group). C RT-PCR was employed to analyze Piezo1 expression in the ileum of both Villin-Piezo1flox/flox mice and WT mice (n = 3 for each group). D Survival for 7 days in WT and CKO mice afer CLP treatment (n = 15 for each group). E Evaluation of intestinal pathological alterations was accomplished through H&E staining. Scale bar 50 μm (n = 4 for each group). Enzyme-linked immunosorbent assay (ELISA) was employed to ascertain the levels of IL-18 (F) and IL-10 (G) in plasma (n = 3 for each group)