Fig. 6

Mechanism of miR-103a-2-5p promotes AML cell apoptosis. THP-1 cells were transfected with control miRNA (miR-NC) or miR-103a-2-5p mimics for 48 h, A Volcano plot showing differential genes of THP-1 cells. B KEGG enrichment analysis of RNA-seq data from THP-1 cells. C Heatmap of the gene expression pattern of the Metabolic pathway in THP-1 cells. D, E Protein level associated with the Nrf2/HO-1 pathway was detected by western blot. F Total GSH in the cells was detected by a GSH assay kit according to the manufacturer’s instructions. G Flow cytometric histograms indicate DCF fluorescence in the AML cell lines. All results are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 vs. control miRNA (miR-NC). Cell experiments were performed three times independently