Fig. 4

Overexpression of miR-103a-2-5p inhibits AML cell proliferation and cell cycle and promotes cell apoptosis in vitro. A After transfection with miR-103a-2-5p mimics or control miRNA (miR-NC) in THP-1, OCI-AML3, OCI-AML2, and MV4-11 cells for 48 h, the qRT-PCR assay was conducted to evaluate transfection efficiency. B After transfection with miR-103a-2-5p mimics or control miRNA (miR-NC) for 48 h, the CCK-8 assay was used to test cell proliferation. C, D Cell cycle distribution was measured by flow cytometry. E, F Levels of the cycle-related proteins, p21, cyclinD1, CDK4, and p53 were detected by western blot analysis. G, H Cell apoptosis was detected by flow cytometry. I, J Levels of the apoptosis-related proteins, cleaved-caspase3, Bax, and Bcl-2 were detected by western blot analysis. All results are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 vs. control miRNA (miR-NC). Cell experiments were performed three times independently