Table 1 The primers for real-time PCR
Genes | Forward Primers (5′-3′) | Reverse Primers (5′-3′) |
|---|---|---|
GAPDH | CAAAATGGTGAAGGTCGGTGT | TGATGTTAGTGGGGTCTCGCT |
HBV-P | CTCTCTTTACGCGGACTCCC | TCTTCTAGGGGACCTGCCTC |
PD-L1 | GCCGAAGTCATCTGGACAAG | TCTCAGTGTGCTGGTCACAT |
CD73 | CCAGTACCAGGGCACTATCTG | TGGCTCGATCAGTCCTTCCA |
CD47 | GGCAATGACGAAGGAGGTTA | ATCCGGTGGTATGGATGAGA |
CD146 | CCAAGGCAACCTCAGCCATGTC | GCAGGGTCTGACACCTCAGCTC |
CD155 | TATCTGGCTCCGAGTGCTTGCC | ACGACGGCTGCAAAAGTGGCG |
ICAM1 | CCCTTCCCCCCAAAACTG | GTCATTGTGAACACTGGCAGAAA |
CD74 | CCAGCGAGGAGCAGAGTCAC | TTATCTCCAACAATGAGCAACT |
IDO | AGCGTCTTTCAGTGCTTTG | GGATTTGACTCTAATGAGCACA |
B7H3 | CACAGGAAGATGCTGCGTCG | CAATGAGACAGACAGACAGC |
CD69 | CATAGCTCTCATTGCCTTATCAGT | CCTCTCTACCTGCGTATCGTTT |