Fig. 3

HBV-DNA-Pol interacts with PARP1. A–C HBV-DNA-Pol interacts with PARP1. A Mass spectrometry predicts a possible interaction of PARP1 with HBV-DNA-Pol. B Immunoprecipitation assays of HBV-DNA-Pol⁺ Huh7 cells were performed with anti-HA antibody and Western blotting with anti-PARP1 and anti-HA antibodies. C Immunoprecipitation assays of HBV-DNA-Pol⁺ Huh7 cells were performed with anti-PARP1 antibody and Western blotting with anti-HA and anti-PARP1 antibodies. D HBV-DNA-Pol co-localizes with PARP1 in the cytoplasm. Immunofluorescence assays of HBV-DNA-Pol⁺ Huh7 cells were performed with the indicated antibodies, then imaged by confocal microscopy. Scale bar, 10 μm. E Schematic diagram of full-length and truncated mutants of HBV-DNA-Pol (a) and PARP1 (b). F Mapping the PARP1-interacting site in HBV-DNA-Pol. Huh7 cells were transfected with the indicated plasmids. After 48 h, cell lysates were immunoprecipitated with anti-PARP1 antibody and then Western blotting with anti-HA and anti-PARP1 antibodies. G Mapping the HBV-DNA-Pol-interacting site in PARP1. HBV-DNA-Pol⁺ Huh7 cells were transfected with the indicated plasmids encoding Flag-tagged full-length PAPR1 or PARP1 deletion mutants. Immunoprecipitation assays were performed with anti-HA antibody and Western blotting with anti-PARP1 and anti-HA antibodies. All results are representative of three independent experiments