Fig. 6

Modulation of HIF-1 signaling by cysteine oxidation in PDAC. A Differential cysteine oxidation of HIF-1 signaling proteins in PANC-1 cells in contrast to the HPDE6c7 cells. HIF-1 signaling proteins with significant alteration of cysteine oxidation levels between the HPDE6c7 and PANC-1 cells were marked by red ovals. Detailed information of their quantitation could be found in Additional file 3: Table S2. The signaling route Fig. was modified from the KEGG database. B, C Decreased oxidation of PHD2 Cys208 site in PANC-1 cells. The oxidation of Cys208 in PHD2 protein was identified by LC–MS/MS (B) and quantitated by label-free strategy (C). D Alteration of PHD2 protein oxidation in cancerous tissues from PDAC patients. Total proteins from cancerous and adjacent tissues were subjected to AMS labeling followed by western blotting against PHD. OX: oxidized; RE: reduced. E The association of PHD protein oxidation and survival times in human PDAC patients. PHD Oca/Tca indicates the ratio of oxidized PHD2 protein levels to total PHD2 protein abundances in pancreatic cancer tissues. Totally 16 PDAC patients were enrolled in this study and used for the survival time analysis. PHD2: prolyl hydroxylase domain 2; **P < 0.01