Fig. 2

Differential protein cysteine oxidation profiles in PDAC cells. A The numbers of cysteine sites and proteins showing differential oxidation levels between HPDE6c7 and PANC-1 cells. B The hierarchical clustering of cysteine sites exhibiting differential oxidation between HPDE6c7 and PANC-1 cells. Increased and decreased oxidation levels were indicated by yellow and green colors, respectively. C A volcano plot of differentially oxidized cysteine residues between HPDE6c7 and PANC-1 cells. Cysteine sites showing up-regulated and down-regulated oxidation were presented as yellow and green points, respectively. D The number of previously reported and newly identified protein cysteine sites characterized in this redox proteomics. Proteins cysteines sites that were identified as S-nitrosylation sites in previous reports were also counted here. E The identification of ENO1 protein oxidation at Cys337 in PANC-1 cells by LC–MS/MS method. The oxidized cysteine residue identified by mass spectrometry was shown as red letter. ENO1: enolase-1