Fig. 1

Proteomic characterization of cysteine oxidation in PDAC cell lines. A The schematic demonstration of iodoTMT-based redox proteomics used for cysteine oxidation profiling in PDAC cells. Protein oxidation in HPDE6c7 and PANC-1 cells were analyzed by the iodoTMT method using three biological replicates, and cysteine relative oxidation levels were calculated by normalizing to their corresponding expressional abundances measured by TMT quantitative proteomics. B A summary of peptides and proteins numbers identified by the iodoTMT-based redox proteomics in HPDE6c7 and PANC-1 cells. C The length and charge distribution of oxidized peptides identified in HPDE6c7 and PANC-1 cells. D Linear correlations between samples used for iodoTMT proteomics by the Pearson’s Correlation Coefficient. E Principal Component Analysis based on the quantitation of protein cysteine oxidation between HPDE6c7 and PANC-1 cell lines. iodoTMT: iodoacetyl tandem mass tag