Fig. 5

Recovery of M28z and MBBz CAR T cells functions after GC exposure. A. Representative flow cytometry analysis used for FACS-based killing assay. B Frequency of viable GFP + MSLN + K562 target cells after 24h incubation with GC-exposed M28z or MBBz CAR T cells. C Frequency of killing reduction of M28z and MBBz induced by 3 exposures with Dx or MP. Relative frequency of LAG-3, PD-1 and TIM-3 in M28z and MBBz CAR T cells after 3 exposures with Dx (D) or MP (E) and 48h rest in a GC-free medium. F Frequency of killing reduction of M28z and MBBz CAR T cells induced by 3 exposures with Dx or MP and 48h rest in a GC-free medium. n = 5 donors. Friedman test with Dunn’s correction was used to compare K562 viability in No GC vs. Dx vs. MP conditions. Student t test was used to compare surface marker expression in GC-exposed vs. GC-rested conditions. Medians are represented. * p < 0.05, **p < 0.01, *** p < 0.001