Fig. 1

Phenotype of CD3 + T cells in CD19, M28z and MBBz CAR T cell products. A Differentiation subsets as defined by CD45RA and CCR7 expression B Representative histogram of glucocorticoid receptor (GR) staining in CD19, M28z and MBBz CAR T cell product in comparison with isotype control staining. C GR expression in CD3 + T cell subsets of CD19, M28z and MBBz CAR products. D GR expression in the naïve and central memory (TCM) subsets of CD19 CAR + and UT of CD4 + (left) and CD8 + (right) T cells. E Comparison of GR expression between CD4 + and CD8 + CD19 CAR T cells in the naïve and TCM memory subsets. F Comparison of GR expression between M28z and MBBz CAR T cells in the CD4 + (left) and CD8 + (right) naïve and TCM memory subsets. MFI: median fluorescence intensity. n = 5 donors for CD19 CAR T cells and n = 4 for M28z and MBBz CAR T cells. Friedman test was used to compare subsets within paired samples, Student t test was used to compare GR expression in different subsets. Medians are represented. *p < 0.05, **p < 0.01