Table 2 Primers for quantitative RT-PCR analysis
Species | Gene | Primer sequence (5'-3') |
|---|---|---|
Rattus norvegicus | G6Pase | Forward: TCAACCTCGTCTTCAAGTGGATT |
Reverse: CTGCTTTATTATAGGCACGGAGCT | ||
Rattus norvegicus | PEPCK | Forward: GGCGGAGCATATGCTGATCC |
Reverse: CCACAGGCACTAGGGAAGGC | ||
Rattus norvegicus | GLUT2 | Forward: CTCGGGCCTTACGTGTTCTTCCTT |
Reverse: TGGTTCCCTTCTGGTCTGTTC | ||
Rattus norvegicus | PPARα | Forward: TCGCAGGAAAGACTAGCAAC |
Reverse: ATGCACAAGGTCTCCATGTC | ||
Rattus norvegicus | PGC-1α | Forward: ATGAGAAGCGGGAGTCTGAA |
Reverse: ACGGTGCATTAATCAATTTC | ||
Rattus norvegicus | BECN1 | Forward: AGTTGCCGTTGTACTGTTCTG |
Reverse: TCAATCTTGCCTTTCTCCAC | ||
Rattus norvegicus | ATG5 | Forward: TGAACGAGAAGCAGAGCCATAC |
Reverse: TTGGATAATGCCATTTCAGG | ||
Rattus norvegicus | LC3 | Forward: CATCAACATTCTGACGGAGCG |
Reverse: GTTGCTTGGCATCAAACACG | ||
Rattus norvegicus | GAPDH | Forward: TCGTGGAGTCTACTGGCGTCTT |
Reverse: CATTGCTGACAATCTTGAGGGAG | ||
Homo sapiens | G6Pase | Forward: GTACAGGGAGAGCTGCAAGG |
Reverse: AGGACGAGGGAGGCTACAAT | ||
Homo sapiens | PEPCK | Forward: CTGCCCAAGATCTTCCATGT |
Reverse: CAGCACCCTGGAGTTCTCTC | ||
Homo sapiens | GLUT2 | Forward: GGCTGAGGAAGAGACTGTGG |
Reverse: ACAGACAGGGACCAGAGCAT | ||
Homo sapiens | AMPK | Forward: CTTTGGCAGTTGCCTACCAT |
Reverse: GGCTTGTCGCCAAATAGAAA | ||
Homo sapiens | PPARα | Forward: CAATGCACTGGAACTGGATG |
Reverse: GCAAATGATAGCAGCCACAA | ||
Homo sapiens | PGC-1α | Forward: CCTTGCAGCACAAGAAAACA |
Reverse: TGACCGAAGTGCTTGTTCAG | ||
Homo sapiens | BECN1 | Forward: GCTGGCACTAGAGGAGGAGA |
Reverse: CGGTTCTTTTCCACGTCTTC | ||
Homo sapiens | ATG5 | Forward: AAAGATGTGCTTCGAGATGTGT |
Reverse: CACTTTGTCAGTTACCAACGTCA | ||
Homo sapiens | LC3 | Forward: CCTTCTTCCTGCTGGTGAAC |
Reverse: CTCGTCTTTCTCCTGCTCGT | ||
Homo sapiens | GAPDH | Forward: GGAGCGAGATCCCTCCAAAAT |
Reverse: GGCTGTTGTCATACTTCTCATGG |