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Fig. 9 | Journal of Translational Medicine

Fig. 9

From: Single-cell transcriptomic sequencing data reveal aberrant DNA methylation in SMAD3 promoter region in tumor-associated fibroblasts affecting molecular mechanism of radiosensitivity in non-small cell lung cancer

Fig. 9

SMAD3 from CAFs induces tumor growth by activating the ITGA6/PI3K/Akt pathway in vivo. Nude mice were treated with H460/oe-NC + Gy, H460/NAF + Gy, H460/oe-SMAD3 + Gy, H460/CAFs + Gy, H460/oe-SMAD3 + sh-NC + Gy, H460/CAFs + sh-NC + Gy, H460/oe-SMAD3 + sh-ITGA6 + Gy and H460/CAFs + sh-ITGA6 + Gy. A Schematic diagram of xenografts in nude mice. B Tumor volume of nude mice. C Tumor weight of nude mice. D Expression of SMAD3 and ITGA6 in tumor tissues of nude mice determined by RT-qPCR. E Western blot of SMAD3, ITGA6 and the PI3K/Akt pathway-related proteins in tumor tissues of nude mice. F Cell proliferation in tumor tissues of mice determined by immunohistochemical staining for Ki67. G Cell apoptosis in tumor tissues of mice determined by TUNEL staining. n = 10. *p < 0.05 vs. H460/oe-NC + Gy or H460/NAF + Gy group. #p < 0.05 vs. H460/oe-SMAD3 + sh-NC + Gy or H460/NAF + sh-NC + Gy group

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Journal of Translational Medicine

ISSN: 1479-5876

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