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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Single-cell transcriptomic sequencing data reveal aberrant DNA methylation in SMAD3 promoter region in tumor-associated fibroblasts affecting molecular mechanism of radiosensitivity in non-small cell lung cancer

Fig. 6

SMAD3 from CAFs promotes activation of the ITGA6/PI3K/Akt pathway in NSCLC cells. A ITGA6 mRNA expression in NSCLC cell lines and 16HBE cells measured by RT-qPCR. B Western blot of ITGA6 and PI3K/Akt pathway-related proteins in NSCLC cell lines and 16HBE cells. C ITGA6 mRNA expression in H460 cells treated with oe-SMAD3 or CAF-CM and in H1229 cells treated with sh-SMAD3 by RT-qPCR. D Western blot of ITGA6 and PI3K/Akt pathway-related proteins in H460 cells treated with oe-SMAD3 or CAF-CM and in H1229 cells treated with sh-SMAD3. *p < 0.05 vs. 16HBE or oe-NC group. n.s. indicates not significant. #p < 0.05 vs. NAF-CM group. &p < 0.05 vs. sh-NC group. Cell experiments were repeated three times

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Journal of Translational Medicine

ISSN: 1479-5876

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