Fig. 7

TS–FMT alleviated gut barrier damage in the DSS-induced UC mice. A Protein expression of Occludin and Claudin-3 in the three groups determined using Western blot; B the relative expression of Occludin was normalized to GAPDH; C the relative expression of Claudin-3 was normalized to GAPDH; D representative images of Control, DSS, S5, TY and TS at the level of ZO-1 by immunofluorescence staining. The image was taken by a confocal microscope with ×100 magnification. Blue: DAPI in the nucleus; Green: ZO-1 in the colon. The data are expressed as the mean ± standard deviation (n = 3). Significance was determined by ANOVA with Tukey’s analysis, p < 0.05, **p < 0.01, ***p < 0.001. (CON–FMT: the feces of the Control group were given to the DSS-treated mice for fecal microbiota transplantation, TS–FMT: the feces of the normal mice orally administered with TS alone were given to the DSS-treated mice for fecal microbiota transplantation)