Fig. 7

Validation of periodontitis-associated differentially expressed genes using qPCR and IHC. A Venn diagram shows the intersection of differentially expressed genes in the six datasets, including IL1β, SRGN, CXCR1, FGR, ARHGEF2, and PTAFR. B Heatmap illustrates differentially expressed genes in the six datasets. C Boxplots show the qPCR results of the six genes (IL1β, SRGN, CXCR1, FGR, ARHGEF2, and PTAFR) in gingival tissues of the periodontitis group and healthy group. D IHC result plots and corresponding histograms show the protein expression levels of IL1β, SRGN, CXCR1 and PTAFR in gingival tissues of periodontitis and healthy groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. IHC: Immunohistochemistry; qPCR: quantitative polymerase chain reaction