Fig. 6

HIF-2α inhibits the expression of TBC1D5 in ccRCC through hsa-mir-7-5p. A The expression of TBC1D5 was significantly elevated in our screening datasets containing the sequencing data of HIF-2α knockdown RCC cell line 786-O. B After the HIF-2α was knocked down, the TBC1D5 protein level was detected using Western Blot. C The sequencing data, TargetScan, and Starbase were used to screen the potential miRNA. D Further screening of two selected miRNA based on the expression and survival patterns. E After the HIF-2α was knocked down, the mRNA level of hsa-mir-7-5p was detected using qRT-PCR. F After using the mimics of miR-7-5p, the expression level of miR-7-5p was detected using PCR, and the TBC1D5 protein level was detected using Western Blot. G After using the inhibitor of miR-7-5p, the expression level of miR-7-5p was detected using PCR, and the TBC1D5 protein level was detected using Western Blot. H ChIP experiment results of potential HIF-2α binding sites in the miR-7-5p promoter are based on the HIF-2α binding sequence. I The luciferase assay results. The truncation of the promoter showed that miR-7-5p bound to the TBC1D5 at position 2912–2918 of TBC1D5 3′ UTR. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NS no significance