Fig. 4

TBC1D5 regulates lipid accumulation by influencing autophagy in renal cancer cells. A GSEA of high and low TBC1D5 expression groups in the TCGA database correlated with multiple autophagy-related pathways. B Correlation between the mRNA expression level of autophagy-related genes (ATG5, BECN1, LC3, and ULK2) and TBC1D5. C After overexpression of TBC1D5, the protein level of LC3 was detected using Western Blot. D, E Autophagy levels were detected using fluorescence and electron microscopy after TBC1D5 overexpression. Scale bar: 100 μm and 500 nm. F The migration ability of A498 and 786-O cells after promoting and inhibiting autophagy treatments was detected using Transwell. Scale bar: 50 μm. G The lipid content of A498 and 786-O cells after promoting and inhibiting autophagy treatment as well as TBC1D5 overexpression was detected using an oil red assay. Scale bar: 100 μm. ****P < 0.0001. CQ chloroquine, RAP rapamycin, OE overexpression