Fig. 3

TBC1D5 acted as a tumor suppressor, suppressed ccRCC progression, and reduced lipid accumulation in ccRCC. A Gene Set Enrichment Analysis (GSEA) of high and low TBC1D5 expression groups in the Cancer Genome Atlas (TCGA) database correlated with metastasis and renal cell carcinoma. B Western blot analysis was used to verify TBC1D5 overexpression at the protein level. C The effect of TBC1D5 overexpression on A498 and 786-O cell proliferation was measured using CCK-8 assay. Five microscopic fields were chosen at random and averaged. D, E The effect of TBC1D5 overexpression on invasion and migration was analyzed using Transwell assays in A498 and 786-O cells, respectively. The histogram represents the relative cell number. Five microscopic fields were selected at random and averaged. Scale bar: 50 μm. F GSEA of high and low TBC1D5 expression groups in the TCGA database correlated with lipid metabolism. G TBC1D5 overexpression significantly decreased lipid droplet accumulation in ccRCC cells tested with oil red staining. Scale bar: 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001