Fig. 2

Relatively low expression of TBC1D5 in ccRCC. A Verification of the TBC1D5 gene signature in five external cohorts from GEO datasets, the mRNA levels of TBC1D5 were compared between tumor tissue and paired paracancer tissue. B The mRNA levels of TBC1D5 in 24 pairs of clinical specimens pathologically diagnosed as ccRCC and paracancer tissues. C TBC1D5 expression was evaluated in six pairs of pathologically diagnosed as ccRCC and paracancer tissues by using Western Blot. D Immunohistochemistry staining of TBC1D5 was performed in the tumor sections of ccRCC and normal tissues and further exhibited by semi-quantification. Scale bar: 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. IOD integral optical density