Fig. 1

Hes and Nar selectively inhibit Drp1. A Ligand-binding pocket of the active site of Drp1. Protein backbone is represented in background as a ribbon, and key protein residues are in blue. (a) Superimposed binding modes of the crystallographic ligand GNP (yellow); Nar (green) and Hes (magenta). The ligands are also shown separately: (b) crystallographic ligand GNP (yellow), (c) NAR and (d) HES. B WB analysis of Drp1 and S616 p-DRP1 was performed in AMO and AMO-BZB cells, 48 h after treatment with increasing doses of Hes, Nar or DMSO as vehicle. GAPDH was used as loading control. C TEM analysis (12000x) of mitochondrial structure and morphology in AMO cell line, 24 h after exposure to 100 µM of Hes or Nar. Representative images are shown; arrows indicate elongated mitochondria