Fig. 4

Cytosolic CDH4 interacts with β-catenin. A An IP assay was performed on TPC-1 cells with expression of His-CDH4, and the immunoprecipitates were separated using SDS-PAGE and stained with Silver. B STRING interaction network was conducted based on CDH4. C Venn diagram showing potential binding genes for CDH4. D IP analyses were performed with TPC-1 and BCPAP cells transiently transfected with His-CDH4 plasmids. E Extracts of PTC cells were immunoprecipitated with anti-β-catenin and control (IgG) antibodies. Co-immunoprecipitated proteins were detected via immunoblotting with their respective antibodies. F Immunofluorescence analysis showing colocalization of β-catenin (red) and CDH4 (green) in PTC cells. Nuclei were counterstained with DAPI (blue). G, H In TPC-1 (G) and BCPAP (H) cells, immunoprecipitation of CDH4 after cytosol-membrane fractionation was detected by western blot. The input was used for protein expression controls, GAPDH cytosol control, or Na + /K + ATPase membrane control. I, J Immunoprecipitation of CDH4 (I) or β-catenin (J) after cytosol-membrane fractionation in TPC-1 cells