Fig. 2

CDH4 promotes the migration, invasion, and angiogenesis of PTC cells. A, B Cellular CDH4 was effectively downregulated using CDH4-targeting siRNAs (A) or shRNA (B). C The expression of LPL was overexpressed in PTC cells by transfecting lentivirus expressing CDH4. D Representative images depicting the localization and intensity of CDH4 in PTC cells with suppressed CDH4 expression. E Immunofluorescence images (left) and quantification (right) of CDH4 in PTC cells with enhanced CDH4 expression. F Typical image showing the morphological changes of TPC-1 and BCPAP cells treated with CDH4-targeting siRNA. G, H Representative images and histogram analysis of cell migration and invasion following overexpression of CDH4 (G) or knockdown of CDH4 (H). I Representative images and quantification of the tube formation of HUVECs cocultured with conditional medium (CoM) derived from TPC-1 and BCPAP cells. *P < 0.05; **P < 0.01; ***P < 0.001