Fig. 1

CDH4 is upregulated in PTC and predominantly localized in the cytoplasm. A Representative IHC images of CDH4 and E-cadherin in PTC tissues and adjacent normal tissues. CM cytomembrane, CP cytoplasm. B The quantification of CDH4 expression in 46 pairs of PTC tissues. C The percentage of CDH4 localized in the cytomembrane or cytoplasm in tumor tissues and adjacent normal tissues. D The expression of CDH4 in 502 PTC tissues and 58 normal thyroid tissues from the TCGA database. E WB analysis of CDH4 and E-cadherin expression in paired PTC tissues and normal tissues. F Detection of the expression level of CDH4 in the cytomembrane or cytoplasm in six PTC tissues. GAPDH and Na + /K + ATPase were used as the internal control for cytosolic proteins or cytomembrane proteins, respectively. G The expression patterns of CDH4 in PTC cell lines and Nthy-ori-1 cells. **P < 0.01; ****P < 0.0001