Fig. 1

Nanostring GeoMx spatial proteomics comparing hippocampal protein expression in Tg-Saline vs. WT-Saline mice. Schematic experimental design for the study (A). Representative images from NanoString GeoMX DSP platform of the Tg-Saline mice showing Aβ plaque-bearing and plaque-free hippocampal regions of interest (ROIs) (the red-boxed region in the thumbnail brain section image in Bi, taken from the Allen Institute, shows the hippocampus which is shown as a high-resolution image in B). Scale bar = 500 μm. Subiculum upper (plaque-bearing dorsal subiculum) (C), CA2 (plaque-free) (D), subiculum lower (plaque-bearing ventral subiculum) (E), and DG (plaque-free) (F) subregions labeled with four morphology markers [Aβ (yellow), Iba1 (red), GFAP (green), and nuclear marker SYTO13 (blue)]. Scale bar = 50 μm. Volcano plots show the magnitude of change (Log2 fold) on the X-axis versus statistical significance on the Y-axis (−Log10(p value)) for 3xTg-Saline vs. WT-Saline comparisons of differentially expressed proteins. Blue dots represent upregulation, red dots represent downregulation, and black dots represent no statistical (NS) change in the subiculum upper (G), subiculum lower (H), CA2 (I), and DG (J) hippocampal regions, respectively. Data from n = 6 mice per group and 4 ROIs per mouse