Fig. 4

Knockdown of Rab32 attenuates PNI-induced pyroptosis in vivo. Peripheral nerves tissue was infected with AAV.shRab32, followed by PNI treatment. These samples were harvested on the 3rd day postoperatively. A and B Expression of NLRP3 and Rab32 mRNA was analyzed by real-time PCR. C The expression of Cleaved-cas1, Rab32, N-GSDMD and NLRP3 protein was evaluated by western blotting, and representative bands are shown. D–G The histograms show the expression of the Rab32, N-GSDMD, Cleaved-cas1 and NLRP3 proteins in according to the semi-quantitative analysis of the bands. H Immunofluorescent analysis revealed the localization of NLPR3 in Schwann cell within the tissue of the peripheral nervous. Red: NLRP3, Green: S100 (Schwann cell marker), and bule: DAPI. Scale bar = 100 μM. **p < 0.01, *p < 0.05