Fig. 3

The impact of MitoQ on pyroptosis in vitro. A The level of ROS was assessed by DCFH-DA staining. B Expression of NLRP3 mRNA was analyzed by real-time PCR. C and D The levels of IL-1β and IL-18 in the culture medium were determined by ELISA. E The expression of Cleaved-cas1, N-GSDMD and NLRP3 protein was evaluated by western blotting, and representative bands are shown. F–H The histograms show the expression of the Cleaved-cas1, N-GSDMD and NLRP3 proteins in according to the semi-quantitative analysis of the bands. I Representative immunofluorescence images showing the location and level of NLRP3 in Schwann cells. J The quantitative analysis of mean fluorescence intensity (MFI) of NLRP3 in each group. Green: NLRP3, blue: DAPI. Scale bar = 20 µm. **p < 0.01, *p < 0.05