Fig. 2

Knocking down of Rab32 significantly reduces the mitochondrial ROS content in Schwann cells in vitro. A Western blotting accessed the expression of Rab32 after Schwann cells were infected with AAV. shRab32 for 24 h. Schwann cells were infected with AAV. shRab32 for a duration of 24 h, followed by induction of cellular pyroptosis using LPS and ATP. **p < 0.01 compared to the shRNA 2 group. *p < 0.05 compared to the shRNA 2 group. B and C Representative immunofluorescence images and quantitative analysis of the level of mitochondrial ROS using MitoSOX staining. Scale bar = 20 µm. **p < 0.01 compared to the LA/shRab32 group. *p < 0.05 compared to the LA/shRab32 group. D The representative images of mitochondrial membrane potential using JC-1 staining. Scale bar = 50 µm. E Quantification of the red-to-green (Percentage of control). Red: JC-1 monomers, green: JC-1 aggregates. **p < 0.01 compared to the LA group. *p < 0.05 compared to the LA group