Fig. 5

Methylation of cg09897064 regulates the polarization of macrophage M2 in vivo, and the growth of tumors caused by mononuclear macrophages. A-B. ZBP1 expression. Western blot was performed to assess the ZBP1 levels in tumor tissue from the experimental groups. C-E. Tumor growth. The tumors formed in mice at the endpoint of mice study.Four groups (n = 6 each) were examined for tumor weight and body weight statistics. E–F. M1/M2 macrophage polarization. An Image-Pro Plus 6.0 was utilized to analyze the expression scores of CD206 and CD86 in tumor tissues, which had been histopathologically and IHC-impaired, and the macrophage polarization was depicted. The mean ± standard deviation of the presented data was found to have statistical significance using appropriate tests(*P < 0.05,**P < 0.01,***P < 0.001)