Fig. 2

Association between cg09897064 methylation on ZBP1 and lung adenocarcinoma prognosis and macrophage polarization. Further analysis of lung adenocarcinoma data from TCGA database revealed the following findings: A. In adjacent tissues, a higher methylation of cg09897064 was seen than in lung adenocarcinoma tissues (P < 0.001). B. Significantly higher mRNA expression of ZBP1 was observed in lung adenocarcinoma tissues compared to neighboring tissues (P < 0.01). C. Patients who had low levels of cg09897064 methylation and high levels of ZBP1 mRNA expression had a significantly improved prognosis compared to thosewith high levels of cg09897064 methylation and low levels of ZBP1 mRNA expression (P < 0.001). D. cg09897064 methylation and tumor immune microenvironment: The "cg09897064 low methylation & ZBP1 mRNA high expression group" showed increased M1 macrophages and decreased M2 macrophages compared to the "cg09897064 high methylation & ZBP1 mRNA low expression group"(P < 0.01)