Fig. 4

The differences in the intestinal tissue transcriptome in the four groups. a Numbers of differentially expressed genes (DEGs) among the control, AI-2, LGG, and LGG+AI-2 four groups. b Venn diagram analysis between the AI-2 vs. control groups and between the LGG+AI-2 vs. LGG groups. c GO_BP enrichment analysis, GO_MF enrichment analysis, and GO_CC enrichment analysis for DEGs except for the AI-2 vs. control groups between the LGG+AI-2 vs. LGG groups. d Heat map analysis of DEGs associated with the cell junction (n = 7 per group). e–g Comparison of Hcar2, cldn3, and cldn4 mRNA expression levels among the four groups (n = 9 per group). DEGs were identified according to fold change≥1.5 and a P-value < 0.05 (DESeq2). Values are presented as the mean ± SEM. Significance was tested using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. BP, biological process; MF, molecular function; CC, cellular component