Fig. 5

Antitumor activity of CAR-NK cells. A The levels of perforin, IFN-γ, and IL-15 secreted by NK and CAR-NK cells as detected by ELISA (1:1 E: T ratio; n = 3; **p < 0.01, ***p < 0.001). B Expression of CD69 on NK/CAR-NK cells as detected by flow cytometry during 4 h of co-culture at a 1:1 E: T ratio. C Intracellular expression of CD107a and IFN-γ on NK/CAR-NK cells as detected by flow cytometry during 4 h of co-culture at a 1:1 E: T ratio