Table 2 Mutations in blood and tumor samples at baseline

Baseline measurement

 n

43

31

 Tumor mutation burden (mutations/mb)

5.4 (3.3–6.7)

4.9 (3.3–6.7)

 KRAS mutation type in tumor

G12D

22 (51.2)

16 (51.6)

G12V

11 (25.6)

7 (22.6)

Others

5 (11.6)

3 (9.7)

Not mutated

5 (11.6)

5 (16.1)

 KRAS mutation in tumor

Yes

38 (88.4)

26 (83.9)

 KRAS mutation abundance in tumor (%)^a

25.8 (12.5–34.4)

23.9 (10.5–36.4)

 KRAS mutation abundance in tumor ≥ 30.0%

Yes

15 (34.9)

10 (32.3)

 KRAS mutation in blood

Yes

32 (74.4)

23 (74.2)

 KRAS mutation abundance in blood (%)^aa

5.8 (1.5–16.7)

4.7 (1.4–13.6)

 KRAS mutation abundance in blood ≥ 5.0%

Yes

16 (37.2)

11 (35.5)

 TP53 mutation in tumor

Yes

30 (69.8)

20 (64.5)

 TP53 mutation abundance in tumor (%)^a

30.8 (15.0–47.7)

30.2 (16.0–51.1)

 TP53 mutation abundance in tumor ≥ 30.0%

Yes

16 (37.2)

10 (32.3)

 TP53 mutation in blood

Yes

27 (62.8)

19 (61.3)

 TP53 mutation abundance in blood (%)^a

7.2 (1.8–12.7)

3.2 (1.9–10.2)

 TP53 mutation abundance in blood ≥ 5.0%

Yes

14 (32.6)

9 (29.0)

 CDKN2A mutation in tumor

Yes

9 (20.9)

7 (22.6)

 CDKN2A mutation abundance in tumor (%)^a

33.4 (20.4–45.9)

33.4 (2.1–45.9)

 CDKN2A mutation abundance in tumor ≥ 30.0%

Yes

5 (11.6)

4 (12.9)

 CDKN2A mutation in blood

Yes

9 (20.9)

7 (22.6)

 CDKN2A mutation abundance in blood (%)^a

6.6 (1.4–8.3)

3.2 (1.4–6.8)

 CDKN2A mutation abundance in blood ≥ 5.0%

Yes

5 (11.6)

3 (9.7)

 SMAD4 mutation in tumor

Yes

7 (16.3)

4 (12.9)

 SMAD4 mutation abundance in tumor (%)^a

19.7 (1.7–39.6)

10.4 (1.2–29.4)

 SMAD4 mutation abundance in tumor ≥ 30.0%

Yes

3 (7.0)

1 (3.2)

 SMAD4 mutation in blood

Yes

7 (16.3)

4 (12.9)

 SMAD4 mutation abundance in blood (%)^a

3.9 (1.5–14.3)

1.9 (1.2–3.1)

 SMAD4 mutation abundance in blood ≥ 5.0%

Yes

3 (7.0)

0 (0.0)

 ARID1A mutation in tumor

Yes

6 (14.0)

4 (12.9)

 ARID1A mutation abundance in tumor (%)^a

11.7 (2.3–21.8)

4.4 (2.2–14.1)

 ARID1A mutation abundance in tumor ≥ 30.0%

1 (2.3)

0 (0.0)

 ARID1A mutation in blood

Yes

4 (9.3)

2 (6.5)

 ARID1A mutation abundance in blood (%)^a

5.7 (1.2–13.4)

1.2 (0.2–2.2)

 ARID1A mutation abundance in blood ≥ 5.0%

Yes

2 (4.7)

0 (0.0)

 BRAF mutation in tumor

Yes

1 (2.3)

1 (3.2)

 BRAF mutation in blood

Yes

2 (4.7)

2 (6.5)

 PI3KCA mutation in tumor

Yes

2 (4.7)

1 (3.2)

 PI3KCA mutation in blood

Yes

0 (0.0)

0 (0.0)

 Number of any mutated genes in tumor

5 (4–7)

5 (4–7)

 Number of any mutated genes in blood

3 (2–5)

4 (2–5)

 Number of mutated driver genes in tumor

2 (2–3)

2 (2–3)

 Number of mutated driver genes in blood

2 (1–3)

2 (1–3)

 Germline mutation

Yes

9 (20.9)

7 (22.6)