Fig. 1

Construction of MSLN-specific CAR-IL-15/IL-15Rα-T cells. A Construction of CAR with IL-15/IL-15Rα. SP represented signal peptide. TM represented transmembrane domain. 15B6 represented CAR constructed with anti-MSLN scFv. 15B6ss represented CAR constructed with anti-MSLN scFv and IL-15/IL-15Rα. C4 represented CAR constructed with anti-MSLN VHH. C4ss represented CAR constructed with anti-MSLN scFv and IL-15/IL-15Rα. B Flow cytometry plots demonstrating CAR expression on untransduced human T cells and CAR-T cells. UTD represented untransduced T cells. C Flow cytometry plots demonstrating IL-15/IL-15Rα on conventional CAR-T cells and CAR-IL-15/IL-15Rα-T cells. D Flow cytometry plots demonstrating the percentage of CAR⁺ and CD8.⁺ T cells in 15B6 and 15B6ss CAR-T after culture with gastric cancer cell line HGC27 for 24 h. Significant difference (*p < 0.05, **p < 0.01) between two groups was calculated using Independent-samples T test by IBM SPSS statistics 20