Fig. 5

Glycine metabolism reprogramming is triggered after knocking down ZSWIM4 in EOC cells. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis in OVCAR8 cells after ZSWIM4 knockdown. B The mRNA expression levels of genes encoding key enzymes for glycine synthesis in vector control and ZSWIM4-knockdown OVCAR8 cells. C, D Protein expression levels of PHGDH and SHMT2 in ZSWIM4-knockdown EOC clones C and ZSWIM4-overexpressing EOC clones D. E Intracellular glycine content of ZSWIM4-knockdown OVCAR8 and SKOV3 cells. F Viability of cells following SHMT2 knockdown in vector control and ZSWIM4-overexpressing EOC cells treated with vehicle or CBP (100 μM) for 72 h; CCK-8 assay. G Rescue experiments using exogenous supplementation of hypoxanthine and GSH in ZSWIM4-knockdown OVCAR8 cells. H ROS levels in vector control and ZSWIM4-knockdown OVCAR8 cells after CBP (50 μM) treatment for 72 h. Representative histograms (upper) and statistical data (mean ± SD) are shown (lower). I Flow cytometry detection of ROS levels in vector control and ZSWIM4-overexpressing OVCAR8 cells treated with vehicle or CBP (50 μM) for 72 h. Representative histograms (upper) and statistical data (mean ± SD) (lower) are shown