Fig. 4

FOXK1-dependent ZSWIM4 transcription contributes to epithelial ovarian cancer (EOC) cell chemotherapy resistance. A FOXK1 protein levels in both EOC cell lines treated with vehicle or CBP (75 μM) for 0, 48, and 72 h. B CUT&Tag-seq analyses of FOXK1 binding peaks on the promoter regions of ZSWIM4 in OVCAR8 and SKOV3 cells. Rep-1, replicated sample-1, Rep-2, replicated sample-2. C Enrichment of ZSWIM4 promoter fragments using an antibody against FOXK1 was assessed by ChIP-qPCR analysis. D Agarose electrophoresis analysis of PCR products of the ZSWIM4 promoter after ChIP-PCR assays. E ZSWIM4 promoter activity in EOC cells transfected with wild-type or mutant plasmids in the presence or absence of 100 μM CBP for 24 h. F FOXK1 knocked down in OVCAR8 and SKOV3 cells. G ZSWIM4 promoter activity in FOXK1 silencing EOC cells treated with vehicle or CBP (75 μM) for 48 h. H Immunofluorescence analysis of ZSWIM4 expression in control and FOXK1-knockdown OVCAR8 cells treated with CBP (75 μM) for 48 h. I ZSWIM4 promoter activity in OVCAR8 cells transfected with wild-type ZSWIM4 promoter-luciferase plasmid with or without FOXK1 co-transfection. The cells were treated with vehicle or CBP (75 μM) for 48 h