Fig. 3

Silencing ZSWIM4 enhances the sensitivity of epithelial ovarian cancer (EOC) cells to CBP. A ZSWIM4 protein expression levels in the vector control and shRNA-mediated ZSWIM4-knockdown EOC clones. Representative flow cytometry histograms (left) and statistical data (mean ± SD) are shown (right). B Killing effects in ZSWIM4-knockdown and shRNA control OVCAR8 cells treated with CBP (100 μM) for 72 h and ZSWIM4-knockdown and shRNA control SKOV3 cells treated with CBP (50 μM) for 72 h; CCK-8 assays. C CBP sensitivity of ZSWIM4-knockdown OVCAR8 and SKOV3 cells. D The statistical data (mean ± SD) of tumorspheres of control and ZSWIM4-knockdown EOC cells incubated in 50 μM CBP for 5 days. E The statistical data (mean ± SD) of apoptosis rate of ZSWIM4-knockdown and control EOC cells with or without CBP (50 μM) treatment for 72 h. F The protein expression levels of cleaved caspase-3 in CBP-treated ZSWIM4-knockdown EOC cells. shC, shCTR. G–I The shRNA control and ZSWIM4-knockdown SKOV3-derived xenograft mice were treated with vehicle control or CBP (20 mg/kg thrice weekly) for two weeks (n = 8/group). Photographs of tumors G. Growth curves of xenograft tumors H. Tumor weight I. Veh, vehicle