Fig. 6

Effects of S. moorei on production of inflammatory factors in AOM-treated mice. A–E Concentrations of TNF-α, IL-6, IL-1β, IL-4 and IL-10 in mice serum. G–K Concentration of TNF-α, IL-6, IL-1β, IL-4 and IL-10 in mice colon tissues. L Representative immunohistochemical staining of CD4⁺ cells in mouse colon tissues. Scale bars = 100 μm. M Relative optical density (OD) of CD4⁺ cells (n = 6). N Representative immunohistochemical staining CD8⁺ cells in mouse colon tissues Scale bars = 100 μm. O Relative OD of CD8.⁺ cells (n = 6). P Representative western blot images of NF-κB, p-NF-κB, IκB, p-IκB in mice colon. Q Quantification of p-NF-κB/NF-κB levels (n = 3). I Quantification of p-IκB/IκB levels (n = 3). S H₂S content in colon tissues of mice (n = 6). Data are presented as the mean ± SD. P values were calculated using one-way ANOVA. *p < 0.05, **p < 0.01