Fig. 4

Effect of S. moorei on AOM-induced progression of APs in mice. A Schematic of the experimental design and timeline of mice models. B FISH analysis of colon sections using S. moorei probes (green) and mucin2 (red) antibodies. C S. moorei promoted dysplasia of colon tissues. Representative histologic images of mouse colon tissues stained with hematoxylin and eosin. Scale bars = 100 μm. D Histology score indicating degree of inflammation and dysplasia in mice (n = 10). E Immunohistochemical analysis of Ki67 + cells in colon tissues. Scale bars = 100 μm. F Quantitative analysis of Ki67 + cells (n = 6). G Representative western blot images of β-catenin, p-Gsk3β, and cyclin D1 protein expression in the mice colon. H Quantification of β-catenin, p-Gsk3β, and cyclin D1 protein levels (n = 3).Data are presented as the mean ± SD. *p < 0.05 and **p < 0.01 (one-way ANOVA)