Fig. 5

BCAT1 acts as an oncogene in CRPC. A BCAT1 protein expression levels in Lenti-vector- or Lenti-BCAT1-infected PC3 and DU145 cells. The lower show the expression intensity normalized to that of β-tubulin. B The proliferation of Lenti-vector- or Lenti-BCAT1-infected PC3 and DU145 cells was measured by a CCK-8 assay. C Lenti-BCAT1 infection increased colony formation in PC3 and DU145 cells. The right show the number of clones. D The migration and invasion of Lenti-vector- and Lenti-BCAT1-infected PC3 and DU145 cells were determined by transwell assays. The right show the quantitation of invasive and migrated cells. E The volume of tumour xenografts in nude mice inoculated with Lenti-BCAT1-infected PC3 cells compared to that of mice inoculated with vector-treated PC3 cells at 21 days (n = 5). F Size (left) and weight (right) of tumour xenografts in nude mice inoculated with Lenti-vector- or Lenti-BCAT1-infected PC3 cells for 21 days (n = 5). G In vivo images of tumours showing increased tumour xenografts and metastatic lesions in nude mice that were orthotopically inoculated with Lenti-BCAT1-infected PC3 cells at 21 days (n = 3). The right panel shows the fluorescence intensity of tumour lesions in each type of nude mouse. H The protein expression levels of BCAT1 in PC3 and DU145 cells transfected with si-NC, si-BCAT1-1 or si-BCAT1-2. The lower show the expression intensity normalized to that of β-tubulin. I The proliferation of si-NC-infected, si-BCAT1-1-infected, and si-BCAT1-2-infected PC3 and DU145 cells was measured by a CCK-8 assay. J The migration and invasion of si-NC, si-BCAT1-1, and si-BCAT1-2 infected PC3 and DU145 cells were determined by transwell assays. The right show the quantitation of invasive or migrated cells. p values are shown for each comparison (^*p < 0.05, ^**p < 0.01)