Fig. 3

BCAT1 is a potential target of HuR in CRPC cells. A Volcano dot plots showing upregulated genes (red dots) and downregulated genes (green dots) in HuR KO1 PC3 and HuR KO4 DU145 cells compared to sgControl cells. B Enrichment analysis of the KEGG pathways showed that the differentially expressed genes (DEGs) in PC3 and DU145 cells with HuR KO were enriched in metabolic pathways. C Venn diagram showing the number of targets and intersecting genes (BCAT1, PIGT, PLA2G4A, MTHFD2L, and NNMT) identified among the DEGs in the metabolic pathway by RNA-seq in both PC3 and DU145 cells. D Relative mRNA levels of BCAT1, PIGT, PLA2G4A, MTHFD2L, and NNMT in PC3 and DU145 cells with HuR KO were measured by qRT-PCR. p values are shown for each comparison (**p < 0.01)