Fig. 1

HuR deficiency attenuates the malignant progression of CRPC cells in vitro. A HuR expression in CRPC tissues compared with LPC tissues in the GSE74367 database. B Representative images showing immunostaining (scale bar: 50 μm) of HuR in human HSPC tissues and CRPC tissues. The dashed square outlines the higher magnification image. The right panel shows the percentages of positive areas (brown) of HuR expression in each type of tissue (n = 5). C Representative qPCR data showing HuR mRNA expression in the different cell lines. D Representative western blots showing HuR expression in the different cell lines. The right panel shows the expression normalized to that of β-tubulin. E The proliferation of sgControl and two HuR KO clones of PC3 and DU145 cells, as measured by CCK-8 assays. F HuR KO inhibited colony formation in PC3 and DU145 cells (left). The right show the number of clones. G Migration and invasion assays of sgControl cells and two HuR KO clones of PC3 and DU145 cells. The right show the corresponding statistical analysis of the number of invasive and migrated cells. p values are shown for each comparison (^*p < 0.05, ^**p < 0.01, ^#p < 0.05, ^##p < 0.01)