Fig. 6

RBBP7 expression is regulated by SP1. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. A. GeneCards is used to explore RBBP7-related transcription factors. B. Correlation analysis between RBBP7 and SP1 expression. C. The JASPAR database was used to predict the putative score for SP1 binding to RBBP7. D–F. SP1 upregulation was established in the Hep3B and was successfully verified by western blotting analysis and RT-qPCR. G–I. The RBBP7 level was determined by western blotting in SP1 overexpressing and control group. J–L. Dual luciferase activity assays were conducted to measure the fluorescence intensity of vector and oeSP1 cells, both with and without mutations in the RBBP7 promoter region, in both 293T and Hep3B cells. M, N. A ChIP-qPCR assay was used to study the enrichment of RBBP7 binding in SP1