Fig. 7

miR-598-3p elicits the anti-Warburg effect and associated tumor metastasis by modulating RMP and IGF1r in GC cells. A, B Wound-healing and transwell assays of hypoxia-cultured SGC-7901 cells transfected with the indicated plasmid. C Western blot analysis of hypoxic SGC-7901 cells transfected with the indicated plasmid. D, E BALB/c nude mice injected via the tail vein or intraperitoneally with SGC-7901 cells expressing the indicated plasmid. The white arrow depicts a metastatic node, n ≥ 5. F, G BALB/c nude mice injected via the tail vein or intraperitoneally with SGC-7901 cells transfected with GFP or miR-598-3p, after which cells were treated with or without UK5099. The white arrow depicts metastatic nodes, n ≥ 5. H Kaplan‒Meier survival curve of BALB/c nude mice injected via the tail vein with SGC-7901 cells transfected with GFP or miR-598-3p after 2 weeks of mice injected with or without UK5099 10 mg/kg/day. N = 10. I, J BALB/c nude mice injected via the tail vein or intraperitoneally with SGC-7901 cells expressing the indicated plasmid, after which cells were treated with or without R406. The white arrow depicts a metastatic node, n ≥ 5. K Kaplan‒Meier survival curve of BALB/c nude mice injected via the tail vein with SGC-7901 cells transfected with GFP, RMP or IGF1r after 2 weeks of injection with or without R406 (1 mg/kg/1 day). n ≥ 10