Fig. 3

Silencing endogenous miR-598-3p promotes the metastasis of gastric cancer cells in vitro and in vivo. A, B Wound-healing assay of SGC-7901 and MKN45 cells transfected with GFP or the sponge of miR-598-3p. C, D Transwell assay of SGC-7901 and MKN45 cells transfected with GFP or the sponge of miR-598-3p. E Real-time PCR analysis of miR-598-3p levels in SGC-7901 and MKN45 cells transfected with GFP or the sponge of miR-598-3p. F, G BALB/c nude mice were injected via the tail vein with SGC-7901 and MKN45 cells transfected with GFP or the sponge of miR-598-3p. The white arrow depicts a metastatic node, n ≥ 5. H, I BALB/c nude mice injected intraperitoneally with MKN45 cells transfected with GFP or the sponge of miR-598-3p. The white arrow depicts a metastatic node, n ≥ 5. J Kaplan‒Meier survival curve of BALB/c nude mice injected via the tail vein with SGC-7901 cells transfected with GFP or the sponge of miR-598-3p, n = 10. K Western blot analysis of SGC-7901 and MKN45 cells transfected with GFP or the sponge of miR-598-3p