Fig. 6

USP14 exhibits tumor-promoting roles by enhancing PFKL-mediated glycolytic metabolism. A HN4 cells stably transfecting with either shCtrl, shUSP14-1#, or shUSP14-1# + PFKL were seeded into 6-well plates at a density of 2000 cells/well. After 10 days, colony formation was detected by crystal violet staining. Data represent the means ± S.D. of three independent experiments. B Wound healing assay was performed on HN4 cells stably transfecting with the indicated plasmids. Images were photographed and analyzed by measurement of the cell-free areas in multiple fields using a service provided by Wimasis. Data represent the means ± S.D. of three independent experiments. C HN4 cells were transfected with the indicated plasmids. Cell migration was investigated using Transwell assay. The numbers of migrated cells per field (mean ± S.D.) from three independent experiments. D, E Cellular glucose consumption and lactate excretion were detected in HN4 cells stably expressing with the indicated plasmids using glucose uptake assay and lactate colorimetric assay, respectively. Data represent the means ± S.D. of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001