Fig. 3

PAOA-mediated intracellular lipid accumulation. A RT-qPCR shown the mRNA expression changes of ACACA in livers. B Western blot analysis of protein levels of ACACA in livers and protein expression was normalized to β-actin. C The effect of different concentrations of PAOA on cell viability after 24 h of incubation. D Representative images of oil red O staining of cells. Scale bars, 200 μm. E The mRNA level of ACACA in cells. Data are the mean ± SEM from 3 independent experiments. ^*P < 0.05 and ^**P < 0.01 compared with the LFD group or Control group