Fig. 11

AT9283 suppresses proliferation of LUAD cells via inhibiting DLGAP5/PLK1 axis. A The 3D docking diagram of the interaction between DLGAP5 protein and AT9283. B The 2D docking diagram of the interaction between DLGAP5 protein and AT9283. C RMSD values for AT9283 during a 50 ns molecular dynamics simulation. D Cells treated with AT9283 (0 nM, 125 nM, 250 nM, 500 nM) and collected 48 h later for detection of DLGAP5 by western blot assay. E–F Cells were treated with AT9283 alone or along with DLGAP5-overexpression plasmids for 48 h. The IC₅₀ of AT9283 was detected by CCK-8 assay. G Cells transfected with AT9283 alone or along with DLGAP5-overexpression plasmid were subjected to clone formation assay. H–I Quantitative analysis of G results and data are presented as mean ± SD, ^**P < 0.01. J Cells transfected with AT9283 alone or along with DLGAP5-overexpression plasmid were collected 48 h later for detection of DLGAP5 and PLK1 by western blot assay