Fig. 10

DLGAP5 promotes LUAD cell proliferation via upregulating PLK1. A GO terms analysis of DLGAP5-related differentially expressed genes (DEGs), shown are top 20 GO terms, color of magenta marked GO terms including PLK1 gene. B KEGG analysis of DLGAP5-related DEGs, color of magenta marked KEGG terms including PLK1 gene. C Cells were transfected with DLGAP5-overexpression plasmid or siRNA against DLGAP5, and collected 48 h later for detection of DLGAP5 and PLK1 by western blot assay. D Cells were transfected with PLK1-overexpression plasmids and collected 48 h later for evaluation of PLK1 overexpression efficiency by western blot assay. E–F Cells transfected with either siDLGAP5 or PLK1-overexpression plasmid, or both were subjected to CCK-8 assay. Data are presented as mean ± SD, ^**P < 0.01. G Cells transfected with either siDLGAP5 or PLK1-overexpression plasmid, or both were subjected to clone formation assay. H–I Quantitative analysis of G results and data are presented as mean ± SD, ^**P < 0.01